Potentiation of Calcium Influx and Insulin Secretion in Pancreatic Beta Cell by the Specific TREK-1 Blocker Spadin

Figure 3

TREK-1 channel current recorded from pancreatic MIN6-B1 cells. (a, b) Whole cell currents measured in MIN6-B1 cells. (a) curves and membrane potentials recorded after 1 h of incubation in control conditions (grey line), after stimulation of TREK-1 current by 10 μM of arachidonic acid (AA) (dark grey line) and in the presence of 1 μM SPA (dark line). (b) Membrane potential mean values obtained from these conditions; the difference between potential values was Δ mV () between control and SPA () and Δ =  mV () between control and glibenclamide (). (c) Membrane potential mean values obtained from three different groups of cells incubated at low (2 mM) and high (20 mM) glucose concentrations in the absence or in the presence of 1 μM ex4 or 1 μM SPA. and .

(a)

(b)

(c)