Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells

Figure 5

(a) Murine GMCs were seeded into 8-well chambered cover glass, grown, and treated as described in the methods. PHB detection was with the same primary antibody as used for IB. PHB detection with an Alexa Fluor 488 conjugated secondary antibody (green). Nuclei were stained with DAPI (blue). Confocal software was used to estimate pixel density in GMC and in nuclei (as defined by DAPI). Nuclei pixel density was subtracted from total density and plotted (b). Differences were estimated by -test with significance at value < 0.05.

(a)

(b)