Influence of Acute High Glucose on Protein Abundance Changes in Murine Glomerular Mesangial Cells

Table 1

Spot	Protein name	Gene product	(HG/NG)	Theoretical		Observed		IPA network	Percent coverage
					pI		pI
1	Not identified		1.23
2	10 kDa heat-shock protein, mitochondrial	CH10_RAT	2.37	10895	8.9	8000	8.3	n/a	77
3	Calpactin I light chain	S10AA_RAT	1.37	11182	6.3	8000	7.4	1	45
4	Macrophage migration inhibitory factor	MIF_RAT	1.34	12640	6.8	9000	7.8	3	26
5	Cofilin-1	COF1_RAT	1.5	18749	8.2	9000	5.3	n/a	34
6	Not identified		1.70
7	Not identified		1.24
8	Profilin-1	PROF1_RAT	1.34	15119	8.5	15000	8.4	1	45
9	Cystatin B	CYTB_RAT	0.82	11303	5.9	10000	6.5	2	80
10	Not identified		0.66
11	Coactosin-like protein	COTL1_MOUSE	1.74	16048	5.3	16000	5.5	1, 2	37
12	Galectin-1	LEG1_RAT	1.23	15189	5.1	15000	5.3	3	61
13	Histidine triad nucleotide-binding protein 1	HINT1_MOUSE	1.39	13882	6.4	11000	7.3	3	49
14	40S ribosomal protein S12	RS12_RAT	1.34	14858	6.8	15000	7.2	2	44
15	Nucleoside diphosphate kinase A (NDK A)	NDKA_RAT	1.68	17296	6.0	16000	6.6	3	39
16	Nucleoside diphosphate kinase B	NDKB_RAT	1.26	17386	6.9	18000	7.6	1	66
17	Not identified		1.89
18	Eukaryotic translation initiation factor 5A	IF5A1_RAT	1.23	17049	5.1	20000	5.6	2	43
	MIR-interacting saposin-like protein	MSAP_MOUSE		21096	5.0	20000	5.6	n/a	29
19	Not identified		1.64
20	Cofilin-1	COF1_RAT	1.63	18749	8.2	21000	8	1	65
21	Cofilin-1	COF1_RAT	1.59	18749	8.2	21000	7.6	1	60
22	Cofilin-1	COF1_RAT	1.57	18749	8.2	21000	7	1	54
23	Not identified		1.61
24	Proteasome subunit alpha type 1	PSA2_RAT	1.54	26024	6.9	23000	7.5	1	47
25	Heat-shock protein beta-1	HSPB1_RAT	2.31	22936	6.1	23000	6.1	1	39
	Phosphoserine phosphatase	SERB_RAT		25180	5.5	23000	6.1	n/a	33
26	14-3-3 protein epsilon	1433E_RAT	1.37	29326	4.6	30000	4.8	1	29
27	Proteasome subunit alpha type 2	PSA1_RAT	1.78	29784	6.2	31000	7.2	1	36
28	Prohibitin	PHB_RAT	2.24	29859	5.6	32000	6.1	2	62
29	Not identified		2.33
30	Proliferating cell nuclear antigen	PCNA_RAT	1.46	29072	4.6	34000	4.9	1	31
31	Heat-shock protein beta-1	HSPB1_MOUSE	0.70	23057	6.1	35000	6.3	n/a	25
32	Annexin A2	ANXA2_RAT	0.83	38939	7.6	40000	7.8	1	56
33	Reticulocalbin 3 precursor	RCN3_HUMAN	1.80	37470	4.7	41000	4.9	2	20
34	Macrophage capping protein	CAPG_RAT	1.53	39060	6.1	41000	6.9	3	20
35	Acetyl-CoA acetyltransferase, cytosolic	THIC_RAT	2.15	41538	6.9	41000	7.7	3	28
36	SUMO-activating enzyme subunit 1	SAE1_RAT	2.15	38945	5.0	41000	5.4	2	54
37	Actin, cytoplasmic-1 (beta-actin)	ACTB_RAT	0.58	42052	5.3	42000	5.7	1	30
	Actin, cytoplasmic-2 (gamma-actin)	ACTG_RAT		42108	5.3	42000	5.7	1	30
38	Not identified		0.6
39	Actin-like protein 3	ARP3_MOUSE	0.53	47783	5.6	50000	6.5	1	38
40	Enolase 1	ENOA_RAT	0.60	47440	6.2	52000	6.7	1	38
	RAB GDP dissociation inhibitor beta	GDIB_RAT		51018	5.9	52000	6.7	n/a	34
41	Not identified		0.37
42	Not identified		0.71
43	Protein disulfide-isomerase A3 (ERp57)	PDIA3_RAT	0.78	57044	5.9	58000	6.4	3	50
44	GRP58	HNRPK_RAT	0.68	51230	5.4	57000	6.3	1	31
45	Not identified		2.21
46	Heterogeneous nuclear ribonucleoprotein L	HNRPL_MOUSE	1.40	60712	6.7	58000	7.8	2	25
47	T-complex protein 1, epsilon subunit	TCPE_RAT	0.78	59955	5.5	58000	6.3	1	34
48	Hsc70/Hsp90-organizing protein	STIP1_RAT	1.16	63158	6.4	65000	7.2	1	38
49	GRP 75	GRP75_RAT	0.82	74097	6.0	75000	6.1	1	31
50	Not identified		1.35
51	RAB 6 interacting protein 2 (ERC protein 1)	GANAB_MOUSE	0.68	107300	5.7	116000	6.4	2	21

Murine GMC protein expression at 2 h culture HG versus 2 h culture NG.