Impact of Pancreatic Rat Islet Density on Cell Survival during Hypoxia

Figure 1

Islet viability. (a) Islet viability at each cell density after 24 hours in culture under both normoxic (PO₂ 160 mmHg) and hypoxic (PO₂ 15 mmHg) conditions. (b) Representative fluorescein diacetate/propidium iodide (FDA/PI) stained images of islets cultured at 150, 300, or 600IEQ/cm² under normoxia and hypoxia. Green staining (FDA) indicates that the cells are alive, while red staining (PI) indicates that the cells are dead. Scale bars = 100 μm. (c) Islet apoptosis at each cell density after 24 hours in culture under both normoxic (PO₂ 160 mmHg) and hypoxic (PO₂ 15 mmHg) conditions. (d) Representative images of TUNEL stained cells cultured at 150, 300, or 600IEQ/cm² under normoxia and hypoxia. Blue staining highlights the cell nuclei (DAPI), and the green staining indicates apoptosis. Scale bars = 50 μm. (e) Caspase-3 activation at each cell density after 24 hours in culture under both normoxic (PO₂ 160 mmHg) and hypoxic (PO₂ 15 mmHg) conditions. compared to the control, compared to 600IEQ/cm² normoxia.

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