Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions

Figure 4

TGF-β1-specific antibody reversed HG-induced p21 gene expression and H3K9me2/3 changes at p21 gene promoter in RMCs. (a) p21 gene mRNA levels in RMCs. Serum-depleted RMCs were treated as described in Materials and Methods. Gene expression was analyzed by RT-qPCR, and results were expressed as fold over NG group (mean ± SEM; compared to NG; compared to HG, ). (b) H3K9me2/3 levels at p21 and CypA gene promoters in RMCs. ChIP assays were performed as described in Materials and Methods. Results were expressed as fold over NG (mean ± SEM; compared to NG; compared to HG, ).

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