Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions

Figure 6

SET7/9 recruitment at p21 gene promoter was enhanced under diabetic conditions, and TGF-β1-specific antibody could reverse HG-induced SET7/9 occupancy in RMCs. (a) SET7/9 recruitment at p21 and CypA gene promoters in glomeruli of control and STZ groups. ChIP assays were performed and results were expressed as described in Materials and Methods (mean ± SEM; compared to control). (b) Reversion of HG-induced SET7/9 enrichment at p21 promoter by TGF-β1-specific antibody. ChIP assays were performed and results were expressed as described in Materials and Methods (mean ± SEM; compared to NG; compared to HG, ).

(a)

(b)