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Journal of Diabetes Research
Volume 2016, Article ID 4208156, 7 pages
Research Article

Longitudinal Frequencies of Blood Leukocyte Subpopulations Differ between NOD and NOR Mice but Do Not Predict Diabetes in NOD Mice

1Institute for Diabetes and Obesity, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
2Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
3German Mouse Clinic, Institute of Experimental Genetics, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
4Institute for Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstraße 30, 81675 Munich, Germany
5German Center for Diabetes Research (DZD), Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
6Forschergruppe Diabetes e.V., Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany
7School of Life Science Weihenstephan, Technical University of Munich, Alte Akademie 8, 85354 Freising, Germany
8Institute for Systemic Inflammation Research, University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany
9DFG Research Center for Regenerative Therapies Dresden, Medical Faculty, Technische Universität Dresden, Fetscherstrasse 105, 01307 Dresden, Germany

Received 6 November 2015; Accepted 31 December 2015

Academic Editor: Paolo Fiorina

Copyright © 2016 Tanja Telieps et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Immune phenotyping provides insight into disease pathogenesis and prognostic markers. Trajectories from age of 4 to 36 weeks were modeled for insulin autoantibodies and for leukocyte subpopulations in peripheral blood from female NOD () and NOR () mice. NOD mice had higher trajectories of insulin autoantibodies, CD4+ and CD8+ T lymphocytes, B lymphocytes, IgD+IgM B lymphocytes, and NK cells and lower trajectories of CD4+CD25+ T lymphocytes, IgM+ B lymphocytes, granulocytes, and monocytes than NOR mice (all ). Of these, only the increased IAA trajectory was observed in NOD mice that developed diabetes as compared to NOD mice that remained diabetes-free. Therefore, the profound differences in peripheral blood leukocyte proportions observed between the diabetes-prone NOD mice and the diabetes-resistant mice do not explain the variation in diabetes development within NOD mice and do not provide markers for diabetes prediction in this model.