Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation

Table 2

Collagen peptide identification from tissue digestion samples.

Col type		# tryp + # semitryp	# nontryp	# Gxx-G
Col-V	α1	2, 0	0	2
	α2	4, 0	0	3
Col-IV	α1	1, 2	3	2
	α2	1, 1	1	1
Col-VI	α1	5, 8	0	0
	α2	9, 1	1	0
	α3	3, 2	1	0

# tryp, # semitryp, and # nontryp indicate the number of tryptic peptides (R/K)↓xxx⋯xxx(R/K)↓xxx⋯, number of semitryptic peptides (R/K)↓xxx⋯ or ⋯xxx(R/K)↓xxx⋯, and number of nontryptic peptides, respectively.
The total number of detected peptides is shown by # tryp + # semitryp + # nontryp.
# Gxx-G can be shared in # tryp, # semitryp, and # nontryp.
Database searches for porcine collagens were based on the following NCBI accession numbers: α1(V): 62461592; α2(V): 157427707; α1(IV): 927191681; α2(IV): 927191683; α1(VI): 92020086, 92020094, and 545895133; α2(VI): 927203606; α3(VI): 92020131 and 927211912.