Transgenerational Glucose Intolerance of Tumor Necrosis Factor with Epigenetic Alteration in Rat Perirenal Adipose Tissue Induced by Intrauterine Hyperglycemia

Figure 7

Methylation analysis and gene expression of Tnf. (a) Bisulfite sequencing was performed on the portion of the promoter regions encompassing −2012 to −1829 relative to the +1 transcription start site of the Tnf gene including 5 CpG sites and −220 to +26 including 8 CpG sites. Each line represents the average methylation level of the sequence of all the clones. CpG sites are shown as blank (unmethylated) or filled (methylated) circles ( per group). (b) The expression of Tnf was identified using real-time PCR (-9). (c) The protein expression of Tnf was identified using Western blotting ( per group). Results are presented as the means ± SEM. versus F1-CN. = 4–9.

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