Transgenerational Glucose Intolerance of Tumor Necrosis Factor with Epigenetic Alteration in Rat Perirenal Adipose Tissue Induced by Intrauterine Hyperglycemia
Methylation analysis and gene expression of Tnf. (a) Bisulfite sequencing was performed on the portion of the promoter regions encompassing −2012 to −1829 relative to the +1 transcription start site of the Tnf gene including 5 CpG sites and −220 to +26 including 8 CpG sites. Each line represents the average methylation level of the sequence of all the clones. CpG sites are shown as blank (unmethylated) or filled (methylated) circles ( per group). (b) The expression of Tnf was identified using real-time PCR (-9). (c) The protein expression of Tnf was identified using Western blotting ( per group). Results are presented as the means ± SEM. versus F1-CN. = 4–9.