Exendin-4 Promotes Survival of Mouse Pancreatic β-Cell Line in Lipotoxic Conditions, through the Extracellular Signal-Related Kinase 1/2 Pathway

Figure 3

The antiapoptotic effect of exendin-4 involves the mitochondrial apoptosis pathway. MIN6 cells were incubated in serum-free DMEM with 0.5% BSA (BSA) or 0.4 mM palmitate added 0.5% BSA (PA), with or without 100 nM exendin-4 (Ex) for 24 h. Protein expression of BCL-2 (a) and BAX (b) was assessed by western blot in MIN6 cells. Values are expressed as relative intensity ratio of BCL-2 or BAX to β-actin and represent the mean ± SE of three independent experiments. , .

(a)

(b)