The Four-Herb Chinese Medicine Formula Tuo-Li-Xiao-Du-San Accelerates Cutaneous Wound Healing in Streptozotocin-Induced Diabetic Rats through Reducing Inflammation and Increasing Angiogenesis

Figure 4

Effects of TLXDS treatment on angiogenesis assessed by the endothelial cell marker CD31 and pericyte marker desmin immunohistochemistry, respectively. (a) Representative CD31 and desmin-staining sections of NC group, DM group, and DM + TLXDS group on day 8 after wounding, respectively. Original magnification 100 and inset magnification 400. (b) Five “hot spots” in each specimen in which the CD31 antibody signal was the most intense were chosen and captured. The number of blood vessels was then counted by two investigators who were blinded to the treatment of the rats using the “manual tagging” feature in Image Pro-Plus software package. (c) Graphic visualization of scores of desmin staining on day 8. for each group. Data are presented as means ± SEM. , compared with NC group; , compared with DM group.

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