Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 1

Reorganization of the F-actin cytoskeleton in podocytes by confocal microscopy. Podocytes under 5.5 mM glucose conditions (column 1) demonstrated that F-actin is distributed as obvious homogenous bundles that traverse the cell along the axis of the podocyte. 30 mM glucose stimulation for 48 hours (column 2) showed an assembly of F-actin in cortical regions, agminated F-actin along the cell periphery, and a slightly diffuse cytoplasmic distribution. Podocytes transfected with Fyn siRNA and then stimulated by 30 mM glucose for 48 hours (column 4) showed reversed F-actin distribution. Pretreatment with 10 μM Y27632 for 30 minutes revealed similar F-actin amelioration (column 6). Podocytes that were exposed to high osmotic pressure (column 3) or transfected with Cont-siRNA (column 6) were considered as controls.