Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 2

Alterations of protein and mRNA levels of synaptopodin in podocytes. Immunofluorescence (a), western blot (b), and real-time PCR (c) revealed that synaptopodin expression was markedly decreased following 48-hour incubation with 30 mM glucose (column 2) when compared with cells incubated in 5.5 mM glucose (column 1) on both protein and mRNA levels. Transfection with Fyn siRNA (column 4) or pretreatment with 10 μM Y27632 for 30 minutes (column 6) partially reversed these alterations. Control podocytes were treated with 5.5 mM glucose plus 24.5 mM mannitol (column 3) or transfected with Cont-siRNA (column 5). Magnification: 400x. Values denote the mean ± SD; versus 5.5 mM glucose and versus 30 mM glucose.

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