Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 4

Alterations of vimentin on protein and mRNA levels in podocytes under different stimulations. Immunofluorescence (a), western blot (b), and real-time PCR (c) presented that vimentin expression was increased markedly following incubation with 30 mM glucose for 48 hours (column 2), and the increase was partially inhibited by transfection with Fyn siRNA for 24 hours (column 4) or pretreatment with 10 μM Y27632 for 30 minutes (column 6). Control podocytes were treated with Cont-siRNA (column 5) or 5.5 mM glucose plus 24.5 mM mannitol (column 3). Magnification: 400x. Values denote the mean ± SD; versus 5.5 mM glucose and versus 30 mM glucose.

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