Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro

Figure 6

Fyn siRNA gene knockdown effects on Fyn expression in podocytes by western blotting and quantitative real-time PCR. A 24-hour transfection with Fyn siRNA notably reduced the expression of Fyn in cells treated with 5.5 mM glucose on both protein and mRNA levels (lane 3). Fyn expression was not significantly altered in podocytes transfected with Cont-siRNA for 24 hours (lane 2) compared with untransfected cells treated with 5.5 mM glucose (lane 1). Values denote the mean ± SD; versus 5.5 mM glucose.