In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications

Figure 4

Pdx1 and NeuroD mRNA expression in islet-derived cells cultured with different media. Analysis of gene expression (mRNA) using qRT-PCR was performed on cells isolated from different pigs and representative results are presented. (a) Islet-derived cells were cultured in media as described in Figure 3(a) and analyzed for the expression of Pdx1 mRNA. (b, c) Islet-derived cells were cultured in media as described in Figure 3(b) and analyzed for the expression of Pdx1 (b) and NeuroD (c) mRNA. The results are the average fold increase for each indicated mRNA compared with the level of this mRNA in adipose-derived stem cells (ADSC) and normalized for the expression of GAPDH.

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