Noninvasive Tracking of Encapsulated Insulin Producing Cells Labelled with Magnetic Microspheres by Magnetic Resonance Imaging

Figure 1

MM labelling. MM are readily taken up by both MIN6 (a) and human islets (b) by simple incubation for 24 hr in culture as evident from the phase contrast (a and b; iv), fluorescent (a and b; v), and histology (a and b; vi) images compared to unlabelled cells (a and b; i, ii, and iii). MM are seen as brown spots (arrows) and are scattered throughout the cytoplasm of both MIN6 (a; vi) and human islets (b; vi). Bar is 50 μm for (a) (i, ii, iv, and v) and (b) (iv and v), 100 μm for (b) (i and ii), 10 μm for (a) (iii and vi), and 20 μm for (b) (iii and vi).

(a)

(b)