Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 5

Cdk5r1 overexpression protects INS-1 β-cells from apoptosis. (a) 832/13 INS-1 cells were transduced with AdCMV-GFP, AdCMV-Cdk5, or AdCMV-Cdk5r1. Twenty-four hours after adenoviral transduction, cells were cultured for 18 hours in the presence of camptothecin, thapsigargin, etoposide, or the vehicle control. Cell counts were completed 18 hours after drug treatment. Cell viability was determined by comparing the untreated population to the treated population. Cells overexpressing Cdk5r1 presented greater viability when treated with thapsigargin or etoposide than control cells. Data represent the of nine independent experiments. p value represents the comparison between Cdk5r1- and GFP-treated 832/13 cells. Cells were transduced with AdCMV-GFP or AdCMV-Cdk5r1 and subsequently treated with camptothecin, thapsigargin, or etoposide. Western blotting for total caspase-3 or cleaved caspase-3 was queried to determine activation of apoptosis pathway. Representative western blot (b) and quantitation (c). Data represent the of four independent experiments. ; .

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