Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 7

Cdk5r1 mediated β-cell proliferation is dependent on the Cdk5 kinase activity. (a) Islets transduced with AdCMV-GFP, AdCMV-Cdk5, or AdCMV-Cdk5r1 were cultured in the presence of ³H-thymidine for 48 hours. Islets were treated with vehicle or 10 μM roscovitine for the final 24 hours of the labeling period. Data represent the of four independent experiments. (b) 832/13 cells were transfected with siCDK5 or siCTRL. Twenty-four hours later cells were transduced with AdCMV-GFP or AdCMV-Cdk5r1. Cdk5 mRNA was measured by RT-PCR, demonstrating a significant decrease in Cdk5 levels with siCDK5 transfection. (c) Proliferation was measured by cell counts, demonstrating that knockdown of Cdk5 causes a significant impairment of Cdk5r1 mediated proliferation. Data represent the of three independent experiments. (c) . p value represents the comparison between Cdk5r1- and GFP-treated islets.

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