Coenzyme Q10 Attenuates High Glucose-Induced Endothelial Progenitor Cell Dysfunction through AMP-Activated Protein Kinase Pathways

Figure 4

CoQ10 attenuates ROS and activates NO and AMPK pathway of EPCs under high glucose conditions. EPCs were incubated with CoQ10 (10 μM) under high glucose conditions. (a) AMPK protein phosphorylation levels of EPCs were analyzed by western blot. Data are mean ± SE; . (b) NO production of EPCs was assessed by staining with NO fluorescent indicator 3-amino,4-aminomethyl-2′,7′-difluorofluorescein (DAF-FM) diacetate (10 μM) for 30 min. (c) ROS production of EPCs was assessed by staining with DCFH-DA (10 μM) for 20 min. The fluorescence intensity was measured using a fluorescent microplate reader. ((d), (e), and (f)) Expressions of Akt protein phosphorylation, eNOS protein phosphorylation, and HO-1 protein were assessed by western blot analysis. Comp C: component C, AMPK inhibitor; L-NAME, NO inhibitor. Data are mean ± SE; ; versus control (5 mM glucose); versus high glucose (HG); versus HG-CoQ10.

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