Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics

Figure 7

Representative MRM results of the ITIH2 analysis in the No DR, Mi NPDR, and Mo NPDR groups. A representative MRM result for ITIH2-peptide (VQSTITSR_2⁺⁺) is shown. The differential concentration range in 60 individual plasmas (a), elution time points for each peptide (b), XIC for the heavy and light peptides of VQSTITSR_2⁺⁺ (c), and the MS/MS spectrum for fragmented peptides (d) are indicated. In our individual SID-MRM analysis, the CV% for retention time (RT) in the No DR, Mi NPDR, and Mo NPDR groups showed ranges of 0.87–8.35, 0.23–4.81, and 0.11–4.92, respectively. All images were extracted using the Skyline program.

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