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Journal of Diabetes Research
Volume 2016, Article ID 6741745, 6 pages
http://dx.doi.org/10.1155/2016/6741745
Research Article

HERG Protein Plays a Role in Moxifloxacin-Induced Hypoglycemia

1Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
2Beijing Key Laboratory of Diabetes Research and Care, Beijing 100730, China

Received 9 July 2015; Accepted 6 August 2015

Academic Editor: Bernard Portha

Copyright © 2016 Hai-Yan Qiu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this study was to investigate the effect of moxifloxacin on HERG channel protein and glucose metabolism. HERG expression was investigated using immunohistochemistry. The whole-cell patch clamp method was used to examine the effect of moxifloxacin on HERG channel currents. A glucose tolerance test was used to analyze the effects of moxifloxacin on blood glucose and insulin concentrations in mice. Results show that HERG protein was expressed in human pancreatic β-cells. Moxifloxacin inhibited HERG time-dependent and tail currents in HEK293 cells in a concentration-dependent manner. The IC50 of moxifloxacin inhibition was 36.65 μmol/L. Moxifloxacin (200 mg/kg) reduced blood glucose levels and increased insulin secretion in wild-type mice at 60 min after the start of the glucose tolerance test. In contrast, moxifloxacin did not significantly alter blood glucose and insulin levels in HERG knockout mice. Serum glucose levels increased and insulin concentrations decreased in HERG knockout mice when compared to wild-type mice. The moxifloxacin-induced decrease in blood glucose and increase in insulin secretion occurred via the HERG protein; thus, HERG protein plays a role in insulin secretion.