HERG Protein Plays a Role in Moxifloxacin-Induced Hypoglycemia
Moxifloxacin-induced inhibition of the HERG channel in HEK293 cells. (a) Cells were voltage-clamped at −80 mV for 1 s, were depolarized from −60 mV to +50 mV for 3 s, and were repolarized to −40 mV for 3 s. HERG currents were recorded in transfected HEK293 cells with and without 100 μmol/L of moxifloxacin. (b) The time-dependent current and peak tail current I-V curves were recorded before and after cell perfusion with 100 μmol/L of moxifloxacin. (c) Cells were depolarized to a voltage of +20 mV for 3 s using a stepped procedure. Peak tail currents were recorded at different moxifloxacin concentrations (10, 100, and 1000 μmol/L). (d) A moxifloxacin concentration-response curve was fitted to a Hill equation, , in which represents 0 tail current inhibition, represents 100% inhibition, represents the IC50 concentration, and represents the Hill slope. , .