Altered Macrophage and Dendritic Cell Response in Mif−/− Mice Reveals a Role of Mif for Inflammatory-Th1 Response in Type 1 Diabetes

Figure 3

Mif−/− mice conserve their healthy anatomy and exhibit limited cellular infiltration into pancreatic islets after STZ administration. Pancreases isolated from Wt or Mif−/− mice were examined via histological analysis of eosin-hematoxylin (E&H) staining to establish the number of pancreatic islets and to determine lymphocyte infiltration (arrowhead in (b)) at 8 weeks after STZ administration. Pancreatic islets from healthy Wt mice (a); Wt mice treated with STZ, WtSTZ (b); healthy Mif−/− mice (c), and Mif−/− mice treated with STZ, Mif−/−STZ (d). Compilation of infiltration stages in the pancreas of Wt and Mif−/− mice after STZ administration (e). Pancreatic islets were scored using the following scale: grade 0 (no insulitis) = 0% infiltration; grade 1 (peri-insulitis) = 1–10% infiltration; grade 2 (moderate insulitis) = 11–<50% infiltration; grade 3 (severe insulitis) = >50% infiltration; or grade 4 (complete insulitis) = complete infiltration. We counted 30–40 islets per experiment using six mice per experimental group, depending on the number of islets that were present in the sections. All data are representative of two independent experiments, from two experiments. , GraphPad Prism software 6.

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