Novel Association of WNK4 Gene, Ala589Ser Polymorphism in Essential Hypertension, and Type 2 Diabetes Mellitus in Malaysia

Figure 1

Detection of Ala589Ser polymorphism of WNK4 gene by PCR-RFLP utilizing 2.0% agarose gel electrophoresis. PCR products were digested by AlwNI. Wild type was cut (into 180 and 112 bp), while variant type remained uncut. Homozygous genotypes are indicated as GG genotypes, heterozygous genotypes are determined as GT genotypes, and TT genotypes show mutant genotypes. M represented a 50 bp DNA ladder plus (Bioline).