Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity

Figure 2

Phenotypic characterisation by flow cytometry of the adipose SVF cells and PBMCs in lean and obese pigs. (a) Gating strategy of the porcine adipose SVF cells from an obese pig. T-cells were defined as CD45⁺CD3⁺. Within the T-cells, CD4⁺, CD8⁺, and CD4⁺CD8⁺ were characterised based on positivity for CD4 and CD8. Macrophages were defined as CD45⁺CD3⁻CD203A⁺, and the M1 and M2 macrophages were determined based on positivity for CD11R3 and CD163, respectively. B-cells were CD45⁺CD3⁻CD203A⁻ and CD21⁺ whereas NK-cells were CD45⁺CD3⁻CD203A⁻ and NKp46⁺. (b–d) Infiltrating leukocyte populations in the adipose tissue within the CD45⁺ population from lean (grey columns) and obese (white columns) pigs. (b) Frequency of the total macrophages (CD203A⁺), classically activated M1 macrophages (CD203⁺CD11R3⁺), and alternatively activated M2 macrophages (CD203⁺CD163⁺). (c) Frequency of natural killer cells (NKp46⁺) and B-cells (CD21⁺). (d) Frequency of the T-helper (CD3⁺CD4⁺), T-cytotoxic (CD3⁺CD8⁺), and double positive T-cells (CD3⁺CD4⁺CD8⁺) within the T-cells. (e–g) PBMCs subpopulation frequencies. (e) Lymphocytes and monocytes. (f) Natural killer cells (CD3⁻NKp46⁺) and B-cells (CD3⁻CD21⁺) populations frequency. (g) Frequency of the T-helper (CD3⁺CD4⁺), T-cytotoxic (CD3⁺CD8⁺), and double positive T-cells (CD3⁺CD4⁺CD8⁺) within the T-cells. Data are mean ± SD. .

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