Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity

Figure 4

Global DNA methylation in PBMCs subpopulations from lean (grey columns and histograms) and obese pigs (open columns and histograms). (a) Representative quantification of 5-methylcytosine levels in lymphocytes and monocytes. (b) Quantification of 5-methylcytosine levels (MFI) in lymphocytes and monocytes. (c) Representative quantification of 5-methylcytosine levels in T-cells (CD3⁺), B-cells (CD21⁺), and natural killer cells (NKp46⁺). (d) Quantification of 5-methylcytosine levels (MFI) in T-cells, B-cells, and natural killer cells. (e) Representative quantification of 5-methylcytosine levels in T-cells subpopulations: T-helper (CD3⁺CD4⁺), T-cytotoxic (CD3⁺CD8⁺), and double positive T-cells (CD3⁺CD4⁺CD8⁺). (f) Quantification of 5-methylcytosine levels (MFI) in T-helper, T-cytotoxic, and double positive T-cells. Data are mean ± SD. . MFI: median fluorescence intensity.