Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy

Figure 3

NAR inhibited deposition of ECM by upregulating let-7a expression. (a) MiRNAs expressions in MMCs treated with NAR. (b) Real-time RT PCR analysis of let-7a in blood and kidney tissues of rats. (c) Real-time RT PCR analysis of let-7a expressions. Data showed let-7a was upregulated or downregulated by let-7a mimics or let-7a inhibitor. Moreover, let-7a was increased in NAR group compared with HG untreated group. (d) Western blot analysis of Col4 and FN proteins in MMCs transferring let-7a mimics or inhibitor with NAR. (e) Quantifications of the western blot bands of Col4 and FN expressions in MMCs transferring let-7a mimics or inhibitor with NAR. (a) Results were normalized with U6 and expressed as mean ± standard deviation ( per group). versus LG group; versus HG group. (b) Results were normalized with U6 and expressed as mean ± standard deviation ( per group). ; ; versus CON group; versus DN group. (c) Results were normalized with U6 and expressed as mean ± standard deviation ( per group). ; versus HG group; versus NAR group. (e) Results were normalized with GAPDH and expressed as mean ± standard deviation ( per group). versus LG group; versus HG group; versus NAR group.

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