Impaired Albumin Uptake and Processing Promote Albuminuria in OVE26 Diabetic Mice

Figure 2

Time course to eliminate TR-albumin fluorescence in FVB and OVE kidneys. Tubule uptake and elimination of injected TR-albumin in FVB and OVE kidneys. Upper panels are from FVB and lower panels from OVE kidneys. Mice were sacrificed at the time points after TR-albumin injection indicated below the images. Initial uptake at 5 min or 30 min is similar in FVB (a, b) and OVE (f, g) tubules. The diabetic and FVB mice differ in that OVE tubules retain TR-albumin for 8 hrs, 1 day, and 3 days (h–j) while no FVB tubules retain TR-albumin at these time points (c–e). Images were made with a 20x objective and the bar in each panel equals 100 μm.