Impaired Albumin Uptake and Processing Promote Albuminuria in OVE26 Diabetic Mice

Figure 3

TR-albumin uptake and endogenous mouse albumin accumulation in OVE tubules. Most OVE tubules that take up TR-albumin, injected 5 min before sacrifice (image (a)), are not the same OVE tubules that accumulate endogenous mouse albumin, shown in image (b) by immunofluorescence (IF) staining for mouse albumin. In the merged image (c) only 3 tubule profiles are yellow indicating costaining for mouse albumin and TR-albumin (shown by the white arrow). Images were made with a 20x objective and the bar in each panel equals 100 μm.

(a)

(b)

(c)