High-Fat Diet Augments VPAC1 Receptor-Mediated PACAP Action on the Liver, Inducing LAR Expression and Insulin Resistance

Figure 4

Effects of LAR knockdown on insulin sensitivity in liver of wild type mice fed HFD. (a) Western blot analysis using anti-LAR and anti-phospho-insulin receptor β-subunit (pIRβ) in the liver of HFD mice at 1 week after injection of AAV-Scr-shRNA or LAR-shRNA. Liver samples were collected at 10 min after insulin injection (0.5 IU/kg). (b) LAR protein levels, relative to β-actin (actin), in the liver. for each group. (c) The intensity of IRβ phosphorylation is expressed by the ratio of phosphorylated over total insulin receptor β-subunit (IRβ) proteins. (d) Blood glucose levels in ITT in HFD mice at 1 week after injection of AAV-Scr-shRNA or LAR-shRNA. Insulin at 0.5 IU/kg body weight was injected to mice fasted for 6 h. All data are presented as means ± s.e.m. .

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