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Journal of Diabetes Research
Volume 2017, Article ID 1328573, 9 pages
Research Article

Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion

1Departamento de Nutrición, Diabetes y Metabolismo, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile
2Centro de Estudios Moleculares de la Célula, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile
3Institute for Research in Dental Sciences, Facultad de Odontología, Universidad de Chile, Santiago, Chile
4Exercise Science Laboratory, School of Kinesiology, Faculty of Medicine, Universidad Finis Terrae, Santiago, Chile
5INSERM UMR1048, Institut des Maladies Métaboliques et Cardiovasculaires, Université Paul Sabatier, Toulouse, France
6Departement de Génétique et Développement, CMU, Université de Genève, Genève, Switzerland
7UMR DIATHEC, EA 7294, Centre Européen d’Etude du Diabète, Université de Strasbourg, Strasbourg, France
8UDA-Ciencias de la Salud, Carrera de Nutrición y Dietética, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile

Correspondence should be addressed to Jose E. Galgani;

Received 29 October 2016; Revised 1 January 2017; Accepted 17 January 2017; Published 14 February 2017

Academic Editor: Stefania Camastra

Copyright © 2017 Maria L. Mizgier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Fasting to postprandial transition requires a tight adjustment of insulin secretion to its demand, so tissue (e.g., skeletal muscle) glucose supply is assured while hypo-/hyperglycemia are prevented. High muscle glucose disposal after meals is pivotal for adapting to increased glycemia and might drive insulin secretion through muscle-released factors (e.g., myokines). We hypothesized that insulin influences myokine secretion and then increases glucose-stimulated insulin secretion (GSIS). In conditioned media from human myotubes incubated with/without insulin (100 nmol/L) for 24 h, myokines were qualitatively and quantitatively characterized using an antibody-based array and ELISA-based technology, respectively. C57BL6/J mice islets and Wistar rat beta cells were incubated for 24 h with control and conditioned media from noninsulin- and insulin-treated myotubes prior to GSIS determination. Conditioned media from insulin-treated versus nontreated myotubes had higher RANTES but lower IL6, IL8, and MCP1 concentration. Qualitative analyses revealed that conditioned media from noninsulin- and insulin-treated myotubes expressed 32 and 23 out of 80 myokines, respectively. Islets incubated with conditioned media from noninsulin-treated myotubes had higher GSIS versus control islets . Meanwhile, conditioned media from insulin-treated myotubes did not influence GSIS. In beta cells, GSIS was similar across conditions. In conclusion, factors being present in noninsulin-stimulated muscle cell-derived media appear to influence GSIS in mice islets.