Research Article
Effect of Human Myotubes-Derived Media on Glucose-Stimulated Insulin Secretion
Figure 4
Effect of conditioned media from noninsulin- and insulin-treated myotubes on glucose-stimulated insulin secretion. Insulin secretion in isolated mice islets (a) and primary rat beta cells (b). Islets and beta cells were incubated with conditioned media from nontreated and insulin-treated myotubes for 24 h before hormone secretion assessment. Controls are unconditioned media without/with added insulin (100 nmol/L). Low glucose = 2.8 mmol/L glucose and high glucose = 16.7 mmol/L glucose. Mean ± SEM. , two-way ANOVA with Tukey post hoc test.
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