Dynamics of Insulin Secretion from EndoC-βH1 β-Cell Pseudoislets in Response to Glucose and Other Nutrient and Nonnutrient Secretagogues

Figure 1

Kinetic profiles of insulin secretion from perifused EndoC-βH1 β-cell pseudoislets in response to a 30 min stimulation with glucose (16.7 mM) (a) () and to the inhibition of glucose-induced (16.7 mM) insulin secretion by mannoheptulose (10 mM) (11–20 min) (b) (). After a 20 min preperifusion phase with basal glucose (3 mM), a 30 min stimulation period with high glucose (16.7 mM) and thereafter a return of the perifusion to basal glucose medium (3 mM) again for another 30 min are depicted. The inset in (a) shows a magnification of the initial 10 min glucose (16.7 mM) stimulation period of the perifusion. Shown are means ± SEM of insulin release rates expressed as ng/min and per 100 pseudoislets (PI).

(a)

(b)