Glycyrrhizic Acid Prevents Diabetic Nephropathy by Activating AMPK/SIRT1/PGC-1α Signaling in db/db Mice

Figure 4

Effect of GA on the expression of α-SMA and TGF-β1 in the renal cortex of db/db mice. (a) Immunohistochemistry was used to detect the expression of α-SMA and TGF-β1. Original magnification: ×400. (b) Western blot assays were used to detect α-SMA and TGF-β1 in mice treated with GA. The proportion of cells with positive protein expression is shown. Histograms indicate the mean ± SD (). β-actin was detected to confirm equal loading. The gray graph indicates the relative level of protein expression in each group.

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