Glycyrrhizic Acid Prevents Diabetic Nephropathy by Activating AMPK/SIRT1/PGC-1α Signaling in db/db Mice

Figure 6

Effect of GA on p-AMPKα, SIRT1, and PGC-1α expression in the kidneys of db/db mice. (a) Immunohistochemistry was used to detect p-AMPKα, SIRT1, and PGC-1α in mice treated with GA. Original magnification: ×400. (b) Western blot analysis was used to detect specific proteins, and the proportion of cells with positive protein expression was determined. Histogram values represent the mean ± SD (). β-actin was detected to confirm equal loading. The gray graphs show the relative protein levels in each group.

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