Diabetic Retinopathy in the Spontaneously Diabetic Torii Rat: Pathogenetic Mechanisms and Preventive Efficacy of Inhibiting the Urokinase-Type Plasminogen Activator Receptor System

Figure 10

Effects of UPARANT on upregulated levels of uPAR and FPRs. (a) Representative blots from SD controls and untreated or UPARANT-treated SDT and STZ rats. (b–e) Protein levels of uPAR (b), FPR1 (c), FPR2 (d), and FPR3 (e) were evaluated by the densitometric analysis of the blots depicted in (a) using β-actin as the loading control. Hyperglycemia enhanced retinal levels of uPAR/FPR proteins in both SDT and STZ rats. In SDT rats, UPARANT reduced the upregulation of uPAR, FPR1, and FPR2 without affecting FPR3. In STZ rats, no effects of UPARANT on protein levels of uPAR or FPRs could be observed. and versus control SD rats; versus untreated SDT rats (one-way ANOVA followed by Newman–Keuls’ multiple comparison posttest; power values: 0.87 (b), 0.99 (c, d), and 0.98 (e)). Each column represents the mean ± SEM of data from 3 independent samples, each containing 1 retina.

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