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Journal of Diabetes Research
Volume 2017, Article ID 3601708, 10 pages
Research Article

Quantitative Proteomic Analysis of Hepatic Tissue of T2DM Rhesus Macaque

Tingfu Du,1,2,3 Shuaiyao Lu,1,3 Qinfang Jiang,1,3 Yun Li,1,3 and Kaili Ma1,2,3

1Center for Drug Safety Evaluation and Research, Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China
2Medical Primate Research Center & Neuroscience Center, Chinese Academy of Medical Sciences, Beijing 100005, China
3Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Diseases, Kunming 650118, China

Correspondence should be addressed to Kaili Ma; moc.liamg@cmuplkm

Received 7 September 2017; Accepted 9 November 2017; Published 14 December 2017

Academic Editor: Janet H. Southerland

Copyright © 2017 Tingfu Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Type 2 diabetes mellitus (T2DM) is a metabolic disorder that severely affects human health, but the pathogenesis of the disease remains unknown. The high-fat/high-sucrose diets combined with streptozotocin- (STZ-) induced nonhuman primate animal model of diabetes are a valuable research source of T2DM. Here, we present a study of a STZ rhesus macaque model of T2DM that utilizes quantitative iTRAQ-based proteomic method. We compared the protein profiles in the liver of STZ-treated macaques as well as age-matched healthy controls. We identified 171 proteins differentially expressed in the STZ-treated groups, about 70 of which were documented as diabetes-related gene in previous studies. Pathway analyses indicated that the biological functions of differentially expressed proteins were related to glycolysis/gluconeogenesis, fatty acid metabolism, complements, and coagulation cascades. Expression change in tryptophan metabolism pathway was also found in this study which may be associations with diabetes. This study is the first to explore genome-wide protein expression in hepatic tissue of diabetes macaque model using HPLC-Q-TOF/MS technology. In addition to providing potential T2DM biomarkers, this quantitative proteomic study may also shed insights regarding the molecular pathogenesis of T2DM.