Prognostic Relevance and Function of MSX2 in Colorectal Cancer

Figure 4

Knockdown of MSX2 expression promoted the cell cycle arrest and apoptosis of CRC cell line. HCT-8 (a, c) and HCT-116 cells (b, d) were harvested after transfection with si-MSX2 and si-NC for 48 h. (a, b) Representative histograms of cell cycle distribution detected by flow cytometry for PI-staining. The green, blue, and yellow peaks represent G1 stage, G2 stage, and S stage, respectively (left panel); data summary of the proportion of cell cycle distribution (right panel) (, , , compared with NC). (c, d) Representative dot-plots of apoptosis detection by flow cytometry for Annexin V-PE and 7-AAD staining (left panel); data summary of the proportion of apoptotic cells with Annexin V⁺7-AAD⁺ cells (right panel) (, , compared with NC). Error bar value represented at least 3 independent experiments (mean ± SD).

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