Hepatic Cholesterol-25-Hydroxylase Overexpression Improves Systemic Insulin Sensitivity in Mice

Figure 1

HFD treatment on mice resulted in decreased hepatic Ch25h protein levels. (a) Ch25h mRNA levels in isolated livers obtained from mice on HFD and control LFD. mRNA levels of hepatic Ch25h levels in LFD mice were xxx-fold higher comparing to mice on HFD. , per group. (b) Immunoblot analysis of Ch25h protein in isolated livers obtained from mice on HFD and control LFD. Mice on HFD showed a clear reduction in liver Ch25h protein levels comparing to the littermate controls. β-Actin was used as a loading control. , per group.

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