Neutrophils Infiltrate the Spinal Cord Parenchyma of Rats with Experimental Diabetic Neuropathy

Figure 2

L-Selectin is increased in the spinal cord of diabetic rats. Cytokine proteome arrays were used to assay levels of 29 cytokines in (a) control, (b) untreated diabetic, and (c) gabapentin-treated diabetic rats. There was no change in the spinal levels of classically proinflammatory cytokines such as (d) TNF-α, (e) IL-1α, or (f) IL-1β (g). However, L-selectin (highlighted in box in (a)–(c)) was significantly increased in diabetic rat lumbar spinal cord, and this increase was ameliorated by treatment with gabapentin ( in a 1-way ANOVA with Bonferroni post hoc tests. Mean optical densities + SD are displayed; ). (h) This was independently confirmed with an ELISA; levels of L-selectin were reduced when diabetic rats were treated with gabapentin ( in a Kruskal-Wallis test with Dunn’s multiple comparison test; -6).

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