Research Article

Sulodexide Protects Renal Tubular Epithelial Cells from Oxidative Stress-Induced Injury via Upregulating Klotho Expression at an Early Stage of Diabetic Kidney Disease

Figure 6

Effect of klotho overexpression on PTEC injury induced by H2O2. (a) The protein levels of cleaved caspase-3 and tubulin are analyzed by Western blot in PTECs after exposure to 0.3 mM H2O2. (b) Densitometry is performed for quantification, and the ratio of cleaved caspase-3 to tubulin is expressed as fold of control. (c, d) Bar graphs show the changes of cell proliferation, characterized by the MTT assay, and HAVCR1 excretions in PTECs after exposure to H2O2. CON = transfected with the control vector; KL = transfected with klotho plasmid. ## and ### versus CON. versus KL.