Diabetes Is Associated with Increased Autoreactivity of Mannan-Binding Lectin

Figure 4

Analysis of retained functionality of AF680 labelled rMBL. (a) rMBL and rMBL labelled with AF680 were analysed for retained binding activity onto a surface of mannan in presence of calcium or EDTA. Values measured in calcium buffer were defined as 100% and subsequent samples were calculated according to these. (b) AF680 labelled rMBL and unlabelled rMBL were analysed for binding activity in the presence of mannose or galactose. Values obtained in the presence of galactose were defined as 100% and subsequent samples were calculated according to these. (c) SDS-PAGE followed by silver staining of rMBL and rMBL AF680 with arrows indicating the distribution of rMBL oligomers. Molecular marker is given in kDa. All values are given in percentage (%) of bound rMBL with values obtained in the presence of galactose defined as 100% bound and subsequent samples calculated according to these. The experiments were repeated twice with similar results.

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