Forkhead Protein FoxO1 Acts as a Repressor to Inhibit Cell Differentiation in Human Fetal Pancreatic Progenitor Cells

Figure 3

Knockdown of FoxO1 results in increased expression of β cell markers. (a) qRT-PCR analysis of FoxO1 mRNA levels in human pancreatic progenitor cells transfected with scramble or FoxO1 siRNA for 48 h. Data were normalized against the level of β-actin and expressed as fold change relative to scramble siRNA group. Values are means ± SEM of 4 independent experiments per each group. . (b) qRT-PCR analysis of β cell markers in human pancreatic progenitor cells transfected with scramble or FoxO1 siRNA for 48 h. Data were expressed as fold change relative to scramble siRNA group and are means ± SEM of 4 to 5 independent experiments. or , or 0.01. (c) Insulin content analysis by ELISA for human fetal progenitor cells, respectively, transfected with FoxO1 siRNA or control siRNA. Values were normalized to total protein content. . (d) Immunohistochemistry with antibodies against FoxO1 (green, dilution of 1 : 100) and insulin (red, dilution of 1 : 100) in the human fetal progenitor cells, respectively, transfected with FoxO1 siRNA (under panels) or control siRNA (upper panels). Arrows indicate the FoxO1 knockdown cells where the insulin was also decreased. Bar = 100 um.

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