Forkhead Protein FoxO1 Acts as a Repressor to Inhibit Cell Differentiation in Human Fetal Pancreatic Progenitor Cells

Figure 5

Constitutive activation of FoxO1 results in decrease of β cell markers. (a) qRT-PCR analysis of FoxO1 mRNA levels in human pancreatic progenitor cells transfected with retrovirus expressing either vector or constitutively active FoxO1 (FoxO1-AAA). Data were normalized against the level of GAPDH and expressed as fold change relative to vector (low panel). Values are means ± SEM of 4 independent experiments per each group. . (b) qRT-PCR analysis of β cell markers in human pancreatic progenitor cells transfected with retrovirus expressing either vector or constitutively active FoxO1 (FoxO1-AAA). Data were expressed as fold change relative to vector and are means ± SEM of 3 to 5 independent experiments per each group. or , or 0.01.

(a)

(b)