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Journal of Diabetes Research
Volume 2017, Article ID 7614685, 12 pages
Research Article

Sympathetic Denervation Accelerates Wound Contraction but Inhibits Reepithelialization and Pericyte Proliferation in Diabetic Mice

Zhifang Zheng,1,2,3 Yu Wan,1,2 Yishu Liu,2,4 Yu Yang,1,2 Jianbing Tang,2 Wenhua Huang,1,3 and Biao Cheng1,2,3,4,5,6

1The Graduate School of Southern Medical University, Guangzhou, China
2Department of Plastic Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China
3Department of Anatomy, School of Basic Medicine Sciences, Southern Medical University, Guangzhou, China
4The Graduate School of Third Military Medical University, Chongqing, China
5Center of Wound Treatment, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, China
6The Key Laboratory of Trauma Treatment & Tissue Repair of Tropical Area, PLA, Guangzhou, China

Correspondence should be addressed to Biao Cheng; moc.361@gnehcoaibgnehc

Received 28 February 2017; Accepted 28 June 2017; Published 24 September 2017

Academic Editor: Ilias Migdalis

Copyright © 2017 Zhifang Zheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Previous studies focused on the effects of sympathetic denervation with 6-hydroxydopamine (6-OHDA) on nondiabetic wounds, but the effects of 6-OHDA on diabetic wounds have not been previously reported. In this study, treated mice received intraperitoneal 6-OHDA, and control mice received intraperitoneal injections of normal saline. Full-thickness wounds were established on the backs of mice. The wounds were sectioned (four mice per group) for analysis at 2, 5, 7, 10, 14, 17, and 21 days after injury. The wound areas in the control group were larger than those in the treatment group. Histological scores for epidermal and dermal regeneration were reduced in the 6-OHDA-treated group on day 21. The mast cells (MCs) in each field decreased after sympathectomy on days 17 and 21. The expression levels of norepinephrine, epidermal growth factor (EGF), interleukin-1 beta, NG2 proteoglycan, and desmin in the treatment group were less than those in the control group. In conclusion, 6-OHDA delays reepithelialization during wound healing in diabetic mice by decreasing EGF, but increases wound contraction by reducing IL-1β levels and the number of MCs. Besides, 6-OHDA led to reduced pericyte proliferation in diabetic wounds, which might explain the vascular dysfunction after sympathetic nerve loss in diabetic wounds.