Suppression of Excessive Histone Deacetylases Activity in Diabetic Hearts Attenuates Myocardial Ischemia/Reperfusion Injury via Mitochondria Apoptosis Pathway

Figure 1

Experimental protocols. Diagram showing the experimental procedures including diabetes induction, ischemia/reperfusion model, TSA and MK-2206 administration, and sacrifice end point. Eight weeks after STZ injection, rats were subjected to 45 min of coronary artery occlusion followed by 3 h of reperfusion. TSA or DMSO was administrated at 48, 24, and 1 h before surgery. MK-2206, wortmannin, or vehicle was applied intravenously 20 minutes before ischemia. TSA: HDAC inhibitor; MK-2206: Akt inhibitor.