db/db Mice Exhibit Features of Human Type 2 Diabetes That Are Not Present in Weight-Matched C57BL/6J Mice Fed a Western Diet

Figure 9

Islet size and insulin-positive area increase in both db/db and WD-fed mice. (a) Immunofluorescent images from pancreatic sections stained for insulin (green), glucagon (red), and DAPI nuclear stain (blue) from C57BL/6J fed WD for 20 weeks (left panel) or 14-week-old db/db mice (right panel). Insulin-positive area (b and c) and calculations of islet area relative to total pancreatic area, that is, islet fraction (d and e) are shown for C57BL/6J mice fed either control or Western diet for 4 or 20 weeks (b, d) and 14-week-old db/+ compared with db/db mice (c, e). Insulin-positive area and islet fraction were quantified using 8 mice per group. Data are shown as mean ± S.E.M. ; ; . CD: control diet; WD: Western diet.

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