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Journal of Diabetes Research
Volume 2017, Article ID 9850398, 13 pages
https://doi.org/10.1155/2017/9850398
Research Article

Lysosomal Exoglycosidase Profile and Secretory Function in the Salivary Glands of Rats with Streptozotocin-Induced Diabetes

1Department of Physiology, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, Poland
2Department of Conservative Dentistry, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland
3Department of Pedodontics, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, Poland
4Department of Cosmetology, Lomza State University of Applied Sciences, Akademicka 1 str, 18-400 Lomza, Poland
5Department of Experimental Pharmacology, Medical University of Bialystok, 37 Szpitalna Street, 15-767 Bialystok, Poland
6Department of Histology and Embryology, Medical University of Bialystok, 13 Waszyngtona Street, Bialystok, Poland
7Department of Emergency Medicine and Disasters, Medical University of Bialystok, 37 Szpitalna Street, 15-767 Bialystok, Poland

Correspondence should be addressed to Mateusz Maciejczyk; moc.liamg@kyzcjeicam.tam

Received 28 July 2017; Revised 6 November 2017; Accepted 23 November 2017; Published 31 December 2017

Academic Editor: Janet H. Southerland

Copyright © 2017 Mateusz Maciejczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Before this study, there had been no research evaluating the relationship between a lysosomal exoglycosidase profile and secretory function in the salivary glands of rats with streptozotocin- (STZ-) induced type 1 diabetes. In our work, rats were divided into 4 groups of 8 animals each: control groups (C2, C4) and diabetic groups (STZ2, STZ4). The secretory function of salivary glands—nonstimulated and stimulated salivary flow, α-amylase, total protein—and salivary exoglycosidase activities—N-acetyl-β-hexosaminidase (HEX, HEX A, and HEX B), β-glucuronidase, α-fucosidase, β-galactosidase, and α-mannosidase—was estimated both in the parotid and submandibular glands of STZ-diabetic and control rats. The study has demonstrated that the activity of most salivary exoglycosidases is significantly higher in the parotid and submandibular glands of STZ-diabetic rats as compared to the healthy controls and that it increases as the disease progresses. Reduced secretory function of diabetic salivary glands was also observed. A significant inverse correlation between HEX B, α-amylase activity, and stimulated salivary flow in diabetic parotid gland has also been shown. Summarizing, STZ-induced diabetes leads to a change in the lysosomal exoglycosidase profile and reduced function of the salivary glands.