Evaluation of Aldose Reductase, Protein Glycation, and Antioxidant Inhibitory Activities of Bioactive Flavonoids in Matricaria recutita L. and Their Structure-Activity Relationship

Table 1

The inhibitory activities of MR crude extract on rat lens aldose reductase (RLAR), DPPH radical scavenging, and advanced glycation end products (AGEs).

Entry	IC50 (μg/mL)^1)
	RLAR	DPPH	AGEs
70% MeOH	4.61 ± 0.29^b	32.39 ± 1.28^b	189.08 ± 4.19^b
Quercetin^2)	3.65 ± 0.10^a	—	—
L-Ascorbic acid^3)	—	6.60 ± 0.33^a	—
Aminoguanidine^4)	—	—	109.10 ± 3.47^a

¹⁾The IC₅₀ values are defined as standard derivation (RSD) of half-maximal inhibitory concentrations obtained from three independent experiments performed in duplicate, and the range of the inhibitor concentrations adopted to evaluate IC₅₀ was prepared as follows: (1) RLAR: 1, 5, and 10 μg/mL; (2) DPPH: 15, 30, and 75 μg/mL; and (3) AGEs: 20, 100, and 200 μg/mL. ^2)–4)Quercetin, L-ascorbic acid, and aminoguanidine are the positive control for RLAR inhibition, DPPH scavenging, and AGEs inhibition. Values within a column marked with different letters are significantly different from each other ().